【 文献重点摘要 】

Mesotherapy/biorevitalization with hyaluronic acid (HA) is a treatment approach currently used for skin rejuvenation. V arious products with a wide range of polycomponent formulations are available on the market. Most of these formulations contain noncross-linked HA in combination with a biorevitalization cocktail, formed by various amounts of vitamins, minerals, amino acids, nucleotides, coenzymes, and antioxidants. Although ingredients are very similar among the different products, in vitro and clinical effects may vary substantially. There is a real need for better characterization of these products in terms of their action on human skin or in vitro skin models. In this study, we analyzed the effect of the RRS® (Repairs, Refills, Stimulates) HA injectable medical device on human skin fibroblasts in vitro. Skin fibroblast viability and its capacity to induce the production of key extracellular matrix were evaluated in the presence of different concentrations of RRS HA injectable. Viability was evaluated through colorimetric MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay, and key extracellular matrix genes, type I collagen and elastin, were quantified by quantitative polymerase chain reaction. Results demonstrated that RRS HA injectable could promote human skin fibroblast viability (+15%) and increase fibroblast gene expression of type I collagen and elastin by 9.7-fold and 14-fold in vitro, respectively. These results demonstrate that mesotherapy/biorevitalization products can, at least in vitro, effectively modulate human skin fibroblasts.

Keywords

mesotherapy, medical device, RRS, collagen, elastin, extracellular matrix

透明质酸的中胚层疗法/生物再生是目前用于皮肤再生的一种治疗方法。市场上有各种多组分配方的产品。这些配方大多含有非交联透明质酸和生物活化鸡尾酒，由不同量的维生素、矿物质、氨基酸、核苷酸、辅酶和抗氧化剂组成。尽管不同产品的成分非常相似，但体外和临床效果可能会有很大差异。就这些产品对人体皮肤或体外皮肤模型的作用而言，确实需要对其进行更好的表征。在这项研究中，我们分析了RRS(修复，补充，刺激)透明质酸注射医疗设备对体外人皮肤成纤维细胞的影响。在存在不同浓度的RRS透明质酸注射液的情况下，评估皮肤成纤维细胞的活力及其诱导产生关键细胞外基质的能力。通过比色MTT (3-[4，5-二甲基噻唑-2-基]-2，5-二苯基四唑鎓溴化物)分析评估生存力，通过定量聚合酶链式反应量化关键细胞外基质基因，ⅰ型胶原和弹性蛋白。结果表明，RRS透明质酸注射液能促进人皮肤成纤维细胞的活力(+15%)，并能使成纤维细胞ⅰ型胶原和弹性蛋白的基因表达分别增加9.7倍和14倍。这些结果表明，至少在体外，中胚层疗法/生物活化产品可以有效地调节人皮肤成纤维细胞。

关键词

中胚层疗法，医疗器械，RRS，胶原蛋白，弹性蛋白，细胞外基质