【 文献重点摘要 】
 

Introduction: Hyaluronic acid (HA)-based fillers are used for various cosmetic procedures. However, due to filler migration and degradation, reinjections of the fillers are often required. Methacrylated HA (MA-HA) can be made into injectable shape-memorizing fillers (three-dimensional [3D] MA-HA) aimed to address these issues. In this study, shape retention, firmness, and biocompatibility of 3D MA-HA injected subcutaneously in mice were evaluated.

Materials and Methods: Fifteen mice, each receiving two subcutaneous injections in their back, were divided into four groups receiving HA, MA-HA, 3D MA-HA, or saline, respectively. Digital imaging, scanning electron microscope (SEM) and in vivo imaging system (IVIS), durometry, and histology were utilized to evaluate in vitro/ vivo degradation and migration, material firmness, and the angiogenic (CD31) and immunogenic (CD45) response of the host tissue toward the injected materials.

Results: Digital imaging, SEM, and IVIS revealed that 3D MA-HA fillers maintained their predetermined shape for at least 30 days in vitro and in vivo. Little volume effects were noted in the saline and other control groups. There were no differences in skin firmness between the groups or over time. Histology showed intact skin architecture in all groups. Three-dimensional MA-HA maintained its macroporous structure with significant angiogenesis at the 3D MA-HA/skin interfaces and throughout the 3D MA-HA. There was no significant inflammatory response to any of the injected materials.

Conclusion: 3D MA-HA showed remarkable tissue compatibility, compliance, and shape predictability, as well as retention, and thus might be suitable for various skin sculpting and soft tissue reconstruction purposes.

Keywords: hyaluronic acid, filler, shape-memorizing, injectable, skin sculpturing, soft tissue reconstruction, scaffold, regeneration, plastic surgery

简介：透明质酸(HA)基填料用于各种美容手术。然而，由于填料的迁移和降解，通常需要重新注入填料。针对这些问题，可将甲基丙烯酸酯化HA(MA-HA)制成可注射形状记忆填料(三维[3D]MA-HA)。本研究评价了3DMA-HA小鼠皮下注射后的形状保持性、坚固性和生物相容性。

材料和方法：15只小鼠背部皮下注射2次，随机分为4组，分别注射HA、MA-HA、3DMA-HA和生理盐水。采用数字成像、扫描电子显微镜(SEM)和体内成像系统(IVIS)、硬度学和组织学等方法评价材料的体内外降解和迁移、材料硬度以及宿主组织对注射材料的血管生成(CD31)和免疫生成(CD45)反应。

结果：数字成像、扫描电镜和IVIS显示3DMA-HA填充物在体外和体内至少保持其预定形状30天。生理盐水组和其他对照组的体积效应很小。在不同组之间或随着时间的推移，皮肤坚固度没有差异。组织学显示各组皮肤结构完整。三维MA-HA保持其大孔结构，在3DMA-HA/皮肤界面和整个3DMA-HA界面均有明显的血管生成。对任何注射的材料都没有明显的炎症反应。

结论：3DMA-HA具有良好的组织相容性、顺应性、形状可预测性和固位性，可用于各种皮肤造型和软组织重建。

关键词：透明质酸，填充物，记忆形状，注射，皮肤雕刻，软组织重建，支架，再生，整形外科